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Computational methods in single molecule localization microscopy
Ovesný, Martin ; Hagen, Guy Michael (advisor) ; Plášek, Jaromír (referee) ; Fliegel, Karel (referee)
Computational methods in single molecule localization microscopy Abstract Fluorescence microscopy is one of the chief tools used in biomedical research as it is a non invasive, non destructive, and highly specific imaging method. Unfortunately, an optical microscope is a diffraction limited system. Maximum achievable spatial resolution is approximately 250 nm laterally and 500 nm axially. Since most of the structures in cells researchers are interested in are smaller than that, increasing resolution is of prime importance. In recent years, several methods for imaging beyond the diffraction barrier have been developed. One of them is single molecule localization microscopy, a powerful method reported to resolve details as small as 5 nm. This approach to fluorescence microscopy is very computationally intensive. Developing methods to analyze single molecule data and to obtain super-resolution images are the topics of this thesis. In localization microscopy, a super-resolution image is reconstructed from a long sequence of conventional images of sparsely distributed single photoswitchable molecules that need to be sys- tematically localized with sub-diffraction precision. We designed, implemented, and experimentally verified a set of methods for automated processing, analysis and visualization of data acquired...
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Hybrid Methods for Nonlinear Least Squares Problems
Lukšan, Ladislav ; Matonoha, Ctirad ; Vlček, Jan
This contribution contains a description and analysis of effective methods for minimization of the nonlinear least squares function F(x) = (1=2)fT (x)f(x), where x ∈ Rn and f ∈ Rm, together with extensive computational tests and comparisons of the introduced methods. All hybrid methods are described in detail and their global convergence is proved in a unified way. Some proofs concerning trust region methods, which are difficult to find in the literature, are also added. In particular, the report contains an analysis of a new simple hybrid method with Jacobian corrections (Section 8) and an investigation of the simple hybrid method for sparse least squares problems proposed previously in [33] (Section 14).
Fulltext: content.csg -  PDF
Plný tet: 0504615-av1 - PDF
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Nonparametric density estimates used for multiple AE source detection
Gális, P. ; Chlada, Milan
Paper deals with the localization of acoustic emission (AE) sources by means of exact geodesic curves on 3D vessels composed of several parametrized surfaces. Precise arrival times of AE events, plugged in DeltaT/DeltaL localization is discussed. Two-dimensional nonparametric (kernel) density estimates are employed to obtain the localization maps on 3-D surfaces and the potential regions of cracks are visibly highlighted. This newly proposed procedure is applied to steam reservoir and other experimental vessels. The results presented are accompanied by thoroughgoing evaluation of their quality and practical usefulness.
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Computational methods in single molecule localization microscopy
Ovesný, Martin ; Hagen, Guy Michael (advisor) ; Plášek, Jaromír (referee) ; Fliegel, Karel (referee)
Computational methods in single molecule localization microscopy Abstract Fluorescence microscopy is one of the chief tools used in biomedical research as it is a non invasive, non destructive, and highly specific imaging method. Unfortunately, an optical microscope is a diffraction limited system. Maximum achievable spatial resolution is approximately 250 nm laterally and 500 nm axially. Since most of the structures in cells researchers are interested in are smaller than that, increasing resolution is of prime importance. In recent years, several methods for imaging beyond the diffraction barrier have been developed. One of them is single molecule localization microscopy, a powerful method reported to resolve details as small as 5 nm. This approach to fluorescence microscopy is very computationally intensive. Developing methods to analyze single molecule data and to obtain super-resolution images are the topics of this thesis. In localization microscopy, a super-resolution image is reconstructed from a long sequence of conventional images of sparsely distributed single photoswitchable molecules that need to be sys- tematically localized with sub-diffraction precision. We designed, implemented, and experimentally verified a set of methods for automated processing, analysis and visualization of data acquired...
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